Although non-invasive prenatal testing (NIPT) is widely used to detect fetal abnormalities, the results of NIPT vary by population, and data for the screening efficiency of NIPT positive predictive value (PPV) from different populations is limited. Herein, we retrospectively analyzed the NIPT results in a large multicenter study involving 52,855 pregnant women. Depending on gestational age, amniotic fluid or umbilical cord blood was extracted for karyotype and/or chromosome microarray analysis (CMA) in NIPT-positive patients, and the PPV and follow-up data were evaluated to determine its clinical value. Among the 52,855 cases, 754 were NIPT-positive, with a positivity rate of 1.4%. Karyotype analysis and/or CMA confirmed 323 chromosomal abnormalities, with a PPV of 45.1%. PPV for trisomy 21 (T21), trisomy 18 (T18), trisomy 13 (T13), sex chromosomal aneuploidies (SCAs), and copy number variations (CNVs) were 78.9, 35.3, 22.2, 36.9, and 32.9%, respectively. The PPVs for T21, T18, and T13 increased with age, whereas the PPVs for SCAs and CNVs had little correlation with age. The PPV was significantly higher in patients with advanced age and abnormal ultrasound. The NIPT results are affected by population characteristics. NIPT had a high PPV for T21 and a low PPV for T13 and T18, and screening for SCAs and CNVs showed clinical significance in southern China.

RESUMO Objetivo: o artigo usa imagens de redes neurais artificiais para explorar os efeitos do exercício aeróbio no ritmo gama do período teta na área CA1 do hipocampo desperto de camundongos APP/PS1/tau e o ritmo gama de baixa frequência da área CA1 do hipocampo do estado de sono Período SWR. Métodos: Camundongos APP/PS1/tau de grau limpo de 6 meses de idade foram divididos aleatoriamente em grupo quieto (AS) e grupo de exercício (AE), os camundongos do grupo controle C57BL/6J foram divididos aleatoriamente em grupo quieto (CS) e grupo de exercício (CE). O grupo AE e o grupo CE realizaram 12 semanas de exercício em esteira, 5d/semana, 60min/d, a primeira carga de exercício de 10min foi de 12m/min, a última de 50min foi de 15m/min e a inclinação da esteira foi de 0 °. Detecção de labirinto de oito braços de mudanças comportamentais em camundongos; tecnologia de gravação in vivo multicanal para registrar os sinais elétricos do estado de vigília e do estado de sono na área CA1 do hipocampo, MATLAB extrai o período de tempo teta do estado de vigília e o período de tempo SWR do estado de sono, método de estimativa de espectro de múltiplas janelas. e análise de densidade espectral de potência. Resultados: 12 semanas de exercícios aeróbicos podem melhorar significativamente a memória de trabalho e a memória de referência do grupo AS, aumentar a energia gama no período teta da área CA1 do hipocampo acordado e a energia gama de baixa frequência na área CA1 do estado de sono período SWR. Conclusões: O exercício aeróbico pode melhorar o estado da rede neural do modelo AD e aumentar a energia gama no período teta da área CA1 do hipocampo e a energia gama de baixa frequência no período SWR é um dos mecanismos da rede neural para seu comportamento geral. Nível de evidência II; Estudos terapêuticos- investigação dos resultados do tratamento.

ABSTRACT Objective: The paper uses artificial neural network images to explore the effects of aerobic exercise on the gamma rhythm of theta period in the awake hippocampal CA1 area of APP/PS1/tau mice and the low-frequency gamma rhythm of the sleep state hippocampal CA1 area SWR period. Methods: Clean grade 6-month-old APP/PS1/tau mice were randomly divided into quiet group (AS) and exercise group (AE), C57BL/6J control group mice were randomly divided into quiet group (CS) and exercise group (CE). The AE group and the CE group performed 12-week treadmill exercise, 5d/week, 60min/d, the first 10min exercise load was 12m/min, the last 50min was 15m/min treadmill slope was 0°. Eight-arm maze detection of behavioral changes in mice; multi-channel in vivo recording technology to record the electrical signals of the awake state and sleep state in the hippocampal CA1 area, MATLAB extracts the awake state theta period and sleep state SWR period, multi-window spectrum estimation method Perform time-frequency analysis and power spectral density analysis. Results: 12 weeks of aerobic exercise can significantly improve the working memory and reference memory of the AS group, increase the gamma energy in theta period of the awake hippocampus CA1 area and the low-frequency gamma energy in the sleep state CA1 area SWR period. Conclusions: Aerobic exercise can improve the neural network state of the AD model and increase the gamma energy in theta period of the hippocampus CA1 area, and the low-frequency gamma energy in the SWR period is one of the neural network mechanisms for its overall behavioral improvement. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Objetivo: El artículo utiliza imágenes de redes neuronales artificiales para explorar los efectos del ejercicio aeróbico en el ritmo gamma del período theta en el área CA1 del hipocampo despierto de ratones APP/PS1/tau y el ritmo gamma de baja frecuencia del área CA1 del hipocampo en estado de sueño. Período de ROE. Métodos: Se dividieron aleatoriamente ratones APP/PS1/tau de 6 meses de edad de grado limpio en grupo tranquilo (AS) y grupo de ejercicio (AE), los ratones del grupo de control C57BL/6J se dividieron aleatoriamente en grupo tranquilo (CS) y grupo de ejercicio (CE). El grupo de EA y el grupo de EC realizaron 12 semanas de ejercicio en cinta rodante, 5 días a la semana, 60 min/d, la primera carga de ejercicio de 10 min fue de 12 m/min, los últimos 50 min fueron de 15 m/min y la pendiente de la cinta fue de 0 °. Detección en laberinto de ocho brazos de cambios de comportamiento en ratones; tecnología de grabación in vivo multicanal para registrar las señales eléctricas del estado despierto y del estado de sueño en el área CA1 del hipocampo, MATLAB extrae el período de tiempo theta del estado despierto y el período de tiempo de SWR del estado de suspensión, método de estimación de espectro de múltiples ventanas Realizar análisis de tiempo-frecuencia y análisis de densidad espectral de potencia. Resultados: 12 semanas de ejercicio aeróbico pueden mejorar significativamente la memoria de trabajo y la memoria de referencia del grupo AS, aumentar la energía gamma en el período theta del área CA1 del hipocampo despierto y la energía gamma de baja frecuencia en el período SWR del área CA1 del estado de sueño. Conclusiones: El ejercicio aeróbico puede mejorar el estado de la red neuronal del modelo AD y aumentar la energía gamma en el período theta del área del hipocampo CA1 y la energía gamma de baja frecuencia en el período SWR es uno de los mecanismos de la red neuronal para su comportamiento general. Nivel de evidencia II; Estudios terapéuticos- investigación de los resultados del tratamiento.

RESUMEN Con el fin de reducir el riesgo de evaluación en la estabilidad de taludes debido a la falta de claridad de los parámetros de cálculo, en este estudio se propone un método de análisis de elementos finitos con base en estadísticas difusas. Basado en el principio del método cuasi-estático y con la ayuda del software multifísico de elementos finitos COMSOL, este trabajo estudia la estabilidad del talud de roca con acumulación de grava en suelo bajo la acción de diferentes aceleraciones sísmicas. Al analizar el desplazamiento, la zona plástica y el factor de seguridad, se analiza la estabilidad del talud del suelo rocoso. Los resultados de la investigación muestran que la falta de claridad de los parámetros mecánicos de la pendiente de la roca y del suelo conducirá a la falta de claridad del desplazamiento de posición y los resultados del análisis de tensión de la pendiente de la roca y el suelo. El análisis de la pendiente de la roca y el suelo con el método de análisis difuso de elementos initos puede fortalecer la comprensión integral del desplazamiento de posición, el estrés y la seguridad de la pendiente de la roca y el suelo por parte de ingenieros y técnicos, y reducir la estabilidad de la pendiente de la roca y el suelo debido a la falta de claridad de los parámetros de cálculo y, hasta cierto punto, evaluar el riesgo cualitativamente.

ABSTRACT In order to reduce the risk of slope stability evaluation due to the fuzziness of calculation parameters, a finite element analysis method of slope stability based on fuzzy statistics is proposed. Based on the principle of quasi-static method and with the help of the finite element software COMSOL multiphysics, this paper studies the stability of the gravel soil accumulation rock slope under the action of different seismic acceleration. By analyzing the displacement, plastic zone and safety factor of the rock soil slope, the stability of the rock soil slope is analyzed. The research results show that the fuzziness of mechanical parameters of rock and soil slope will lead to the fuzziness of position displacement and stress analysis results of rock and soil slope, and the analysis of rock and soil slope with the method of fuzzy finite element analysis can strengthen the comprehensive understanding of position displacement, stress and safety of rock and soil slope by engineers and technicians, and reduce the stability of rock and soil slope due to the fuzziness of calculation parameters to a certain extent Evaluate the risk qualitatively.

Abstract Vancomycin is a first-line drug for treating methicillin-resistant Staphylococcus aureus. Thrombocytopenia is a rare adverse reaction to vancomycin treatment, and there are no reports of vancomycin-induced thrombocytopenia (VIT) in infants. We describe the case of a 3-month-old girl who was diagnosed with purulent meningitis. After 13 days of treatment with vancomycin, her platelet count reduced to 8 × 109/L. Vancomycin was discontinued, and intravenous methylprednisolone was administered. The platelet count returned to normal after 4 days. Patients, especially young children, receiving vancomycin for a long clinical course should undergo careful monitoring of laboratory indicators and blood tests.

ABSTRACT The purpose of this study was to investigate the effects of gosling quality on their growth parameters, intestinal villus morphology, blood biochemistry, and nonspecific pathological lesions from 0 to 4 weeks old. Seventy-two goslings were randomly distributed into 12 pens, with each pen containing three males and three females in a completely randomized design of two control variables, including healthy and weak goslings. Healthy goslings all shared the same characteristics, such as good features with no abnormalities and full absorption of yolk sac. Albeit lighter body weight (<85 g/bird) and incomplete absorption of the yolk sac were found in the weak geese, no obvious disease was exhibited in them. Post mortem examination revealed that the incidence of nonspecific pathological lesions of the 4 week-old healthy and weak gosling groups were 25.0 and 50.0%, respectively. Nonspecific pathological changes of 4 week-old goslings in the liver, ileum, lung, and heart were 16.7, 12.5, 8.3, and 4.2%, respectively. The healthy goslings also had higher total cholesterol, high-density lipoprotein, and low-density lipoprotein than weak goslings. Separately raising weak goslings from 0 to 4 weeks old could increase the survival rate and reduce economic losses of feeding.

ABSTRACT The purpose of this study was to demonstrate the in vitro free radical scavenging activity and antioxidant capacity in solid-state fermented wheat bran and its potential modulation of antioxidative molecular targets in chicken peripheral blood mononuclear cells (PBMC). After solid-state fermentation of wheat bran by white rot fungi for 12 d, the scavenging action of the fermented wheat bran extracts was 1, 1-diphenyl-2-picrylhydrazyl (DPPH), and the free radicals increased significantly, approximately 1.5-fold. Trolox equivalent antioxidant capacity of 1 mg/mL fermented wheat bran extracts was increased from 100 to 150 mM trolox antioxidant capacity after 12 d of fermentation. Moreover, the extracts exhibited 50% of the chelating capacity observed for ferrous iron (Fe2+) after fermenting for 12 d. In vitro, and under the stimulus of fermented wheat bran, the antioxidant gene expression (GST, HO-1, Nrf2, and GCLC genes) of PBMC was more than double that of the PBS, ascorbic acid, and unfermented wheat bran. The expression of fermented wheat bran was the lowest for the NOX1 and ROMO1 genes. Solid-state wheat bran fermented by white rot fungi can increase the scavenging action of DPPH, the trolox equivalent antioxidant capacity, and the chelating capacity of ferrous iron; in addition, in vitro, it can regulate the expression of antioxidant molecular targets in chicken PBMC.

ABSTRACT The objective of this study was to determine the effect of spent mushroom compost (SMC) meal on the growth performance and meat characteristics of geese. The SMC extracts contained 2.49±0.62 mg gallic acid equivalent/g dry weight (DW) and 1.08±0.15 mg quercetin equivalent/g DW of the total phenolic and flavonoid contents. A total of 120 White Roman geese, aged five weeks, were randomly distributed among 12 pens and fed a grower diet ad libitum during the growing period, with each pen containing five males and five females in a completely randomized design. Each treatment comprised three pens (total of 30 geese), including control (corn-soybean meal); supplementation with 5% SMC meal (5% SMC); supplementation with 10% SMC (10% SMC); and supplementation with 15% SMC (15% SMC), for eight weeks. The results revealed that the body weight of the 15% SMC group was significantly lower than that of the control group at the age of 12 weeks. There were no significant effects among the groups on feed conversion ratio or intake. The malondialdehyde content of the serum in the 15% SMC group was lower than that of the control group at week 12. There were no significant effects among the groups for blood biochemical parameters in grower geese at week 12. The color values of meats in the SMC groups were higher than those obtained with control group. The flavor and acceptability score of meats in the 5% SMC group were significantly higher than for the 15% SMC and control group. Supplementation with SMC at 5% in the diet has no adverse effects on the growth performance of grower geese. However, SMC meal at 5% in the diet favorably affects sensory attributes (meat flavor and acceptability).